Single pyrimidine discrimination during voltage-driven translocation of osmylated oligodeoxynucleotides via the α-hemolysin nanopore

• Yun Ding and
• Anastassia Kanavarioti

Beilstein J. Nanotechnol. 2016, 7, 91–101, doi:10.3762/bjnano.7.11

• above detection limits, may be further optimized, and clear the way for nanopore-based sequencing using osmylated DNA. Keywords: DNA sequencing; α-hemolysin; ion-channel measurements; nanopore; osmium tetroxide bipyridine; osmylated oligos; osmylation; single-stranded DNA (ssDNA); Introduction

• column, and Figure 3), as well as the absence of unreacted OsBp. The modified oligos were evaluated via the α-HL pore using an instrument custom made for the Chemistry Department of the University of Utah equipped with a glass pore membrane (GNM, see Experimental section). Ion channel measurements were

• current and slowed down the translocation of the modified oligo. However the markedly different ion-channel measurements observed between dA10dT(OsBp)dA9 and dA10dC(OsBp)dA9 were unexpected (see Graphical Abstract, Figure 4, and Table 2). It turns out that dT(OsBp) yields more relative current obstruction